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Emerging extended-spectrum beta-lactamases in Proteus mirabilis
Laura Pagani1, Roberta Migliavacca, Lucia Pallecchi
1Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sezione di Microbiologia, Università di Pavia, Italy.
Abstract:
Beta-lactamase production was detected in 147 (52%) of 282 consecutive nonduplicate Proteus mirabilis isolates obtained over a 1-year period from the S. Matteo Hospital of Pavia (northern Italy). Seventy isolates (48% of the beta-lactamase producers) were found to produce extended-spectrum beta-lactamases (ESBLs), identified as PER-1 (first report in this species) and TEM-52 in 52 and 18 isolates, respectively. Analysis of clonal diversity of the ESBL producers suggested different spreading patterns for the two ESBL determinants.
Insights
Extended-spectrum beta-lactamases (ESBLs) were found in 48% of Proteus mirabilis isolates. The study identified PER-1 and TEM-52 as the main ESBL types, noting distinct spreading patterns for these antimicrobial resistance determinants.
Area of Science:
- Microbiology
- Infectious Diseases
- Antimicrobial Resistance
Background:
- Proteus mirabilis is a common cause of healthcare-associated infections.
- Beta-lactamase enzymes confer resistance to beta-lactam antibiotics, a critical class of antimicrobials.
- Extended-spectrum beta-lactamases (ESBLs) represent a significant challenge in treating bacterial infections due to their broad activity.
Purpose of the Study:
- To investigate the prevalence and types of beta-lactamase production in Proteus mirabilis isolates.
- To identify specific ESBLs, including extended-spectrum beta-lactamases (ESBLs), in clinical isolates.
- To analyze the clonal diversity and potential dissemination patterns of ESBL-producing Proteus mirabilis.
Main Methods:
- Antimicrobial susceptibility testing was performed on 282 Proteus mirabilis isolates.
- Beta-lactamase production was phenotypically screened.
- Isolates positive for beta-lactamase production were further characterized for ESBL production.
- Specific ESBLs (PER-1 and TEM-52) were identified.
Main Results:
- Beta-lactamase production was detected in 52% (147/282) of the Proteus mirabilis isolates.
- Of the beta-lactamase producers, 48% (70/147) were identified as producing ESBLs.
- The identified ESBLs were PER-1 (52 isolates) and TEM-52 (18 isolates), with PER-1 being reported for the first time in this species.
- Analysis suggested different clonal spreading patterns for the PER-1 and TEM-52 ESBL determinants.
Conclusions:
- A significant proportion of Proteus mirabilis isolates in this northern Italian hospital produce beta-lactamases, including clinically relevant ESBLs.
- The emergence of PER-1 in Proteus mirabilis highlights the evolving landscape of antimicrobial resistance.
- Understanding the epidemiology and dissemination of ESBL-producing Proteus mirabilis is crucial for infection control and treatment strategies.