Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiplexed protein profiling on microarrays by rolling-circle amplification.

Barry Schweitzer1, Scott Roberts, Brian Grimwade

  • 1Molecular Staging, Inc., Suite 701, 300 George Street, New Haven, CT 06511, USA.

Nature Biotechnology
|March 30, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Leveraging time-series point clouds for dynamic crop canopy monitoring: Quantifying phenotypic variability and assessing leaf-level photosynthetic contributions.

Plant phenomics (Washington, D.C.)·2026
Same author

Early time to first recurrence predicts multiple recurrent ischemic strokes: A 2.5-year prospective cohort study.

Neuroscience·2026
Same author

A phosphorylated variant of the mast/stem cell growth factor receptor KIT is upregulated in dorsal root ganglia of Friedreich ataxia.

Histology and histopathology·2026
Same author

Hypoplastic Vertebral Artery Contributes to Stroke by Promoting Progression of Basilar Artery Bending.

Brain and behavior·2026
Same author

Immune divergence in rainbow trout lines selectively bred for F. psychrophilum resistance or susceptibility revealed by challenge with Weissella tructae.

Fish & shellfish immunology·2026
Same author

Global health authorities review and approval trends for CMC post approval changes 2018-2024.

PDA journal of pharmaceutical science and technology·2026
Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026
Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026
See all related articles

This study introduces rolling-circle amplification (RCA) coupled with universal antibodies for enhanced immunoassay sensitivity in proteomics. This method enables precise, large-scale cytokine measurement for biological discovery.

Area of Science:

  • Proteomics
  • Immunology
  • Biotechnology

Background:

  • Fluorescent-sandwich immunoassays on microarrays are valuable for proteomics due to readily available reagents and simple, scalable, and reproducible assays.
  • Achieving adequate sensitivity and specificity in immunoassays requires a general amplification method.

Purpose of the Study:

  • To describe the coupling of isothermal rolling-circle amplification (RCA) to universal antibodies for immunoassay signal amplification.
  • To demonstrate the utility of RCA-enhanced immunoassays for simultaneous measurement of multiple cytokines on microarrays.

Main Methods:

  • Coupling of isothermal rolling-circle amplification (RCA) to universal antibodies.
  • Simultaneous measurement of 75 cytokines on glass arrays using RCA for signal amplification.

Related Experiment Videos

  • Utilizing a 51-feature RCA cytokine glass array to analyze human dendritic cell (DC) secretion.

    • Main Results:

    • RCA-based immunoassays achieved femtomolar sensitivity, high specificity, a 3-log quantitative range, and economy of sample consumption.
    • Lipopolysaccharide (LPS) induced secretion of inflammatory cytokines including macrophage inflammatory protein (MIP)-1beta, interleukin (IL)-8, and interferon-inducible protein (IP)-10.
    • Tumor necrosis factor-alpha (TNF-alpha) treatment induced macrophage-derived chemokine (MDC), thymus and activation-regulated chemokine (TARC), soluble interleukin 6 receptor (sIL-6R), and soluble tumor necrosis factor receptor I (sTNF-RI).

    Conclusions:

    • RCA-based immunoassays offer a sensitive and specific method for multiplexed cytokine analysis.
    • This approach is suitable for studying cellular responses, such as those induced by LPS and TNF-alpha in dendritic cells.
    • The scalability of RCA microarrays presents a tractable approach for comprehensive proteomic surveys.