Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lysozyme in calcifying tissues.

K E Kuettner, R Eisenstein, N Sorgente

    Clinical Orthopaedics and Related Research
    |October 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Cartilage lysozyme regulates calcification by interacting with proteoglycans. This interaction likely initiates and controls the cartilage mineralization process, impacting bone development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The possible function of lysozyme in cartilage metabolism.

    Annals of the rheumatic diseases·2014
    Same author

    Self-rated sleep characteristics and bother from nocturia.

    International journal of clinical practice·2012
    Same author

    LYSOZYME IN EPIPHYSEAL CARTILAGE : IV. Embryonic Chick Cartilage Lysozyme-Its Localization and Partial Characterization.

    The Journal of cell biology·2009
    Same author

    ENZYME ACTIVITIES IN CHICK EMBRYONIC CARTILAGE : Their Subcellular Distribution in Isolated Chondrocytes.

    The Journal of cell biology·2009
    Same author

    Direct measurement of the 7Be solar neutrino flux with 192 days of borexino data.

    Physical review letters·2008
    Same author

    [Ankle chondrocytes are more resistant to Interleukin-1 than chondrocytes derived from the knee].

    Der Orthopade·2006

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Connective Tissue Research

    Background:

    • Lysozyme is synthesized by chondrocytes and secreted into the cartilage extracellular matrix.
    • Cartilage lysozyme levels vary, increasing near the cartilage-bone junction and decreasing with parathyroid extract, suggesting a role in bone metabolism.
    • Low cartilage lysozyme in rickets and its increase with vitamin D indicate a link to calcification.

    Purpose of the Study:

    • To investigate the role of cartilage lysozyme in the regulation and initiation of cartilage calcification.
    • To explore the interaction of lysozyme with cartilage components, particularly proteoglycans and hyaluronic acid.

    Main Methods:

    • Analysis of lysozyme distribution and levels in cartilage, including the epiphyseal growth plate.

    Related Experiment Videos

  • In vitro studies examining the interaction of lysozyme with cartilage proteoglycans, chondroitin sulfate, and hyaluronic acid.
  • Comparison of mammalian cartilage lysozyme with hen egg white lysozyme activity.

    • Main Results:

    • Cartilage lysozyme appears bound or inactivated by an inhibitor in the native tissue.
    • Aggregated proteoglycans inhibit mineral accretion in vitro, but mammalian cartilage lysozyme can inactivate this inhibition.
    • This inactivation is likely mediated by an interaction with hyaluronic acid, leading to disaggregation.

    Conclusions:

    • Cartilage lysozyme plays a crucial role in regulating and initiating cartilage calcification.
    • Lysozyme's interaction with aggregated proteoglycans and hyaluronic acid is key to its function in mineralization.
    • Further research is warranted to fully elucidate the mechanisms of lysozyme in skeletal development.