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Updated: Jul 19, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
The role of configurational entropy in biochemical cooperativity
Sutjano Jusuf1, Patrick J Loll, Paul H Axelsen
1Department of Pharmacology, University of Pennsylvania, School of Medicine, Philadelphia, Pennsylvania 19104-6084, USA.
Abstract:
Cooperativity is a common biochemical phenomenon in which two or more otherwise independent processes are thermodynamically coupled. Because cooperative processes are usually attended by changes in molecular conformation, thermodynamic coupling is usually attributed to an enthalpy-driven mechanism. In the family of glycopeptide antibiotics that includes vancomycin, however, cooperative phenomena occur that cannot be explained by conformational change. In this communication, we demonstrate that cooperativity in these systems can arise solely from changes in vibrational activity.
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