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Updated: Aug 9, 2026

Eukaryotic Polyribosome Profile Analysis
Published on: June 15, 2010
Translational readthrough of the PDE2 stop codon modulates cAMP levels in Saccharomyces cerevisiae
Olivier Namy1, Guillemette Duchateau-Nguyen, Jean-Pierre Rousset
1Laboratoire de génétique moléculaire de la traduction, Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
Abstract:
The efficiency of translation termination in yeast can vary several 100-fold, depending on the context around the stop codon. We performed a computer analysis designed to identify yeast open reading frames (ORFs) containing a readthrough motif surrounding the termination codon. Eight ORFs were found to display inefficient stop codon recognition, one of which, PDE2, encodes the high-affinity cAMP phosphodiesterase. We demonstrate that Pde2p stability is very impaired by the readthrough-dependent extension of the protein. A 20-fold increase in readthrough of PDE2 was observed in a [PSI+] as compared with a [psi-] strain. Consistent with this observation, an important increase in cAMP concentration was observed in suppressor backgrounds. These results provide a molecular explanation for at least some of the secondary phenotypes associated with suppressor backgrounds.
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