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Related Experiment Videos

Determining dermal absorption parameters in vivo from tape strip data.

Micaela B Reddy1, Audra L Stinchcomb, Richard H Guy

  • 1Chemical Engineering and Petroleum Refining Department, Colorado School of Mines, Golden 80401, USA.

Pharmaceutical Research
|April 6, 2002
PubMed
Summary

Tape stripping experiments measuring dermal absorption can be impacted by chemical diffusion. Accounting for diffusion during stripping ensures accurate permeability coefficient calculations, especially when the stripping time is less than 0.2 times the lag time.

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Area of Science:

  • Dermal absorption and toxicology
  • Skin penetration studies
  • Pharmacokinetics and biophysics

Background:

  • Tape stripping (TS) is a common in vivo method to evaluate dermal absorption through the stratum corneum (sc).
  • Chemical diffusion into the sc during the time required for tape stripping (tTS) can significantly influence experimental outcomes.
  • Understanding these diffusion effects is crucial for accurate interpretation of TS data.

Purpose of the Study:

  • To investigate how chemical diffusion into the stratum corneum affects tape stripping experiment results.
  • To examine the impact of diffusion during stripping on the interpretation of dermal absorption data.
  • To determine conditions under which diffusion alters TS experimental outcomes.

Main Methods:

  • Human dermal absorption of 4-cyanophenol (4CP) was studied using tape stripping.

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  • Mathematical models were developed to quantify the effects of diffusion on parameter estimation.
  • Permeability coefficients were calculated with and without accounting for diffusion during stripping.

    • Main Results:

    • When diffusion during stripping was considered (tTS > tlag), the calculated permeability coefficient for 4CP (P(sc,v)) aligned with literature values (0.0019 cm/h).
    • Excluding diffusion from the model resulted in a 70% underestimation of the P(sc,v).
    • Chemical concentrations in TSs are influenced by diffusion, but this effect is minimal if tTS < 0.2 tlag and exposure time is > 0.3 tlag.

    Conclusions:

    • Diffusion during tape stripping can alter chemical concentrations in collected tape strips.
    • Accurate estimation of dermal absorption parameters requires accounting for diffusion during the stripping process.
    • Specific conditions (tTS < 0.2 tlag and exposure > 0.3 tlag) minimize the impact of diffusion on TS concentrations.