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Mutational analysis of the bglH catabolite-responsive element (cre) in Lactobacillus plantarum
Rosangela Marasco1, Lidia Muscariello, Manuela Rigano
1Facoltà di Scienze MM FF NN, Università degli Studi del Sannio, via Caio Ponzio Telesino 11, 82100, Benevento, Italy.
Abstract:
Catabolite repression of the Lactobacillus plantarum bglH gene is mediated by the catabolite control protein A (CcpA). Here we show that the binding site for the protein is a catabolite-responsive element (cre) sequence overlapping the start site of transcription. Two single and one double base substitutions in the cre sequence caused derepression of a plasmid-borne bglH-cat transcriptional fusion in L. plantarum cells grown on glucose. Analysis of the single mutations indicates that the G and C nucleotide residues in positions 2 and 13, respectively, of the 14-bp cre sequence are required for catabolite repression.