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Systematic optimisation of high-performance liquid chromatographic separation by varying the temperature, gradient,
Matthias Otto1, Anke Schirmer, Ute Claussnitzer
1Institute of Analytical Chemistry, Freiberg University of Mining and Technology, Germany.
Analytical and Bioanalytical Chemistry
|April 9, 2002
Abstract:
Optimisation of the separation of a synthetic drug mixture by HPLC is performed by changing both continuous variables, i.e. mobile phase composition and temperature, and categorical variables, here the stationary phase. The retention of solutes is described on the basis of a general linear model in which the different columns are modelled by indicator variables. From the solute-specific retention models the global separation optimum is evaluated on the basis of multidimensional window diagrams using relative retentions of all peak pairs as the figure-of-merit.