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Related Experiment Videos

Carmine-picroindigocarmine: an alternative multiple staining method.

Andres Arend1, Ivo Kolts

  • 1Department of Anatomy, University of Tartu, Biomedicum, Estonia.

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
|April 9, 2002
PubMed
Summary

This study revisits Carmine-picroindigocarmine, a historic multiple staining method. The technique offers vibrant, stable tissue coloration and serves as a valuable alternative for histological analysis.

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Area of Science:

  • Histology
  • Pathology
  • Biomedical Sciences

Background:

  • The Carmine-picroindigocarmine stain, developed by Professor Harry Kull at the University of Tartu, is a long-standing multiple staining technique.
  • This method combines copper carmine, picric acid, and indigocarmine to produce distinct and colorful histological results.

Purpose of the Study:

  • To describe the Carmine-picroindigocarmine multiple staining method.
  • To highlight its effectiveness in differentiating various tissue and cellular structures.
  • To explore its compatibility with additional stains, such as resorcin-fuchsine.

Main Methods:

  • Utilizing a combination of copper carmine, picric acid, and indigocarmine for tissue staining.
  • Observing the differential coloration of nuclear structures, cytoplasm, collagen fibers, cartilage matrix, muscle tissues, and erythrocytes.

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  • Assessing the stability of the stain and its capacity for combination with other dyes, specifically resorcin-fuchsine.

    • Main Results:

    • Nuclear structures are stained red; cytoplasm varies from yellow to green.
    • Collagen fibers appear blue, hyaline cartilage matrix is greyish-blue, and muscle tissues range from brownish-red to brownish-green.
    • Erythrocytes are yellow; squamous epithelia are red, with darker red in horny layers. Nail plate and hairs stain bright yellow.
    • The stain is stable and compatible with additional dyes, such as resorcin-fuchsine, which stains elastic fibers violet, clearly distinguishing them from blue-stained collagen fibers.

    Conclusions:

    • Carmine-picroindigocarmine staining provides bright, colorful, and stable results for histological samples.
    • The method effectively differentiates various tissue components, offering an aesthetic and informative alternative to other multiple staining techniques.
    • Combining Carmine-picroindigocarmine with resorcin-fuchsine enhances differentiation, particularly for elastic fibers, without compromising the original staining characteristics.