[Modulation of endothelin-1 on pulmonary surfactant synthesis of cultured alveolar type II cells]

The effects of endothelin-1(ET-1) on pulmonary surfactant(PS) synthesis of cultured AT II cells were observed. The results showed that: 1. ET-1(10(-11)-10(-8) mol.L-1) enhanced PS synthesis of cultured AT II cells in a dose-dependent manner. 2. The minimum effective concentration of ET-1 enhanced PS synthesis of cultured AT II cells was higher than that of lung explants. 3. ETA antagonist BQ123 decreased the effect of ET-1 on PS synthesis, but ETB antagonist BQ788 did not change the effect. 4. ET-1 (10(-12) and 10(-10) mol.L-1) had no effect on the proliferation of AT II cells. These results confirmed that ET-1 can enhance directly and indirectly on the PS synthesis of AT II cells mediated via ETA receptor. The effect of ET-1 on PS synthesis was not induced by changing the number of AT II cells.