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Reference intervals for thyroid hormones on the architect analyser.

Walter Hubl1, Jürgen Schmieder, Eberhard Gladrow

  • 1Institut für Klinische Chemie und Laboratoriumsmedizin, Krankenhaus Dresden-Friedrichstadt, Dresden, Germany. hubl-wa@khdf.de

Clinical Chemistry and Laboratory Medicine
|April 10, 2002
PubMed
Summary

This study established population-specific reference intervals for key thyroid hormones, including thyroid stimulating hormone (TSH) and free thyroxine (FT4), using the Architect i2000 analyser. Findings highlight the need for localized thyroid hormone reference ranges.

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Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Laboratory Medicine

Background:

  • Accurate thyroid hormone reference intervals are crucial for diagnosing thyroid dysfunction.
  • Existing reference intervals may not be universally applicable due to population variations.
  • The Architect i2000 analyser is a widely used platform for thyroid hormone testing.

Purpose of the Study:

  • To establish reference intervals for thyroid stimulating hormone (TSH), free thyroxine (FT4), free triiodothyronine (FT3), total thyroxine (TT4), and total triiodothyronine (TT3).
  • To evaluate these intervals using the Architect i2000 analyser (Abbott).
  • To assess the influence of age, sex, and health status on thyroid hormone concentrations.

Main Methods:

  • Serum samples were collected from three groups: healthy adults (n=217), ambulatory euthyroid patients (n=323), and hospitalized euthyroid patients (n=490).

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  • Exclusion criteria included oral contraceptive use, hormone replacement therapy, history of thyroid disorders, and severe non-thyroidal illness.
  • Thyroid hormone levels were measured using the Architect i2000 analyser.

    • Main Results:

    • Established reference intervals for TSH, FT4, FT3, TT4, and TT3 in healthy adults.
    • Identified significant sex-based differences in FT4, FT3, and TT4 levels.
    • Observed age-related variations in TSH, FT3, and TT3, with lower concentrations in older individuals (>50 years) for TSH and higher for FT3/TT3 in younger individuals.
    • Found no significant differences between healthy subjects and ambulatory euthyroid patients.
    • Determined reference intervals differed considerably from international values, emphasizing the need for population-specific ranges.

    Conclusions:

    • The study successfully established population-specific reference intervals for key thyroid hormones on the Architect i2000 analyser.
    • Age and sex significantly influence thyroid hormone levels, necessitating consideration in clinical interpretation.
    • These findings underscore the importance of utilizing localized reference ranges for accurate thyroid function assessment.