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Possible function of outward potassium currents in isolated Deiters' cells of guinea pig cochlea
1Department of Otorhinolaryngology, Union Hospital, Tongji Medical University, Wuhan 430022, China. yangjun67@263.net
Objective:
To study potassium currents in isolated Deiters' cells of guinea pig cochlea and explore possible function of potassium current in Deiters' cell.
Methods:
The whole cell patch clamp recording technique was used to study potassium currents of Deiters' cells in normal external solution and solutions with different K(+) concentrations. We also studied the effects on reversal potentials and outward potassium currents.
Results:
Isolated Deiters' cells possess voltage dependent, outwardly rectifying ion channels, which are K(+) selective. 50 mmol/L K(+) and 150 mmol/L K(+) in external solution reduced I(K-max) from (10.06 +/- 2.2) nA (n = 13) in normal external solution to (6.43 +/- 1.67) nA (n = 6, P < 0.05) and (5.49 +/- 1.33) nA (n = 6, P < 0.05), respectively. While the amplitude of tailcurrents decreased from (468.76 +/- 61.76) pA in 5 mmol K(+) external solution to (224.74 +/- 35.89) pA (P < 0.05) in 50 mmol/L K(+) and to (-911.59 +/- 78.17) pA (P < 0.01) in 150 mmol/L K(+) external solution.
Conclusions:
Outwardly rectifying potassium in Deiters' cells could buffer extracellular K(+) in the small space between Deiters' cells and outer hair cells or neural fibers and participate in the diffusion of K(+) from endolymph to perilymph.
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