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Studies on the DNA content of breast carcinoma cells with neuroendocrine differentiation
Genyou Yao1, Jilin Zhou, Zhongsheng Zhao
1Department of Pathology, Zhejiang University Medical School, Hangzhou 310031, China. yaogy@zjuem.zju.edu.cn
Objective:
To make quantitative analysis of DNA content of breast cancer with neuroendocrine (NE) cells and its significance.
Methods:
Using MIPS-III image analyzer, DNA content and 9 parameter measurements of the tumor nuclei were made in both NE positive (17) and negative (64) breast carcinomas.
Results:
Out of 81 breast carcinomas, 17 cases were NE positive while 64 cases were NE negative. In the NE (+) cases, the integral optic density, mean optic density, DNA index, DNA stemlines peak, > 5c aneuploidy cells and the rate of aneuploidy cells were all lower than those in the NE negative breast carcinoma cases (P < 0.01). The positive rates of NE cells were 32.5% and 7.9% in grade I - II breast carcinomas and in grade III breast carcinomas respectively with significant difference between the two groups (P < 0.01).
Conclusion:
Our study shows that NE (+) breast carcinomas have lower DNA parameters than NE (-) breast carcinomas, suggesting that NE (+) breast carcinomas have lower malignancy.