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Mannitol transport in Streptococcus mutans.

J H Maryanski, C L Wittenberger

    Journal of Bacteriology
    |December 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Streptococcus mutans utilizes a hexitol-inducible phosphotransferase system to metabolize mannitol. This system, dependent on phosphoenolpyruvate, is specific to the substrate and can be enhanced by a heat-stable component.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Molecular Biology

    Background:

    • Streptococcus mutans is a key bacterium in dental caries development.
    • Understanding its metabolic pathways, particularly carbohydrate transport, is crucial for developing preventive strategies.

    Purpose of the Study:

    • To demonstrate and characterize the hexitol-inducible phosphoenolpyruvate-dependent phosphotransferase system in Streptococcus mutans.
    • To investigate the substrate specificity and regulatory components of this system.

    Main Methods:

    • Preparation of cell-free extracts from Streptococcus mutans strains grown on various hexitols (mannitol, sorbitol) and glucose.
    • Enzymatic assays measuring the conversion of hexitol to hexitol-phosphate using phosphoenolpyruvate as the phosphate donor.
    • Partial purification of a heat-stable stimulatory component from glucose-grown cells.

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    Main Results:

    • A phosphoenolpyruvate-dependent phosphotransferase system for mannitol was identified in Streptococcus mutans, active when cells were grown on mannitol.
    • This system exhibited substrate specificity, with mannitol and sorbitol phosphotransferase activities detected only when cells were grown on the respective hexitol.
    • A heat-stable, low-molecular-weight component from glucose-grown cells partially purified and found to stimulate the mannitol phosphotransferase system.
    • Divalent cations Mn2+ and Ca2+ could partially substitute for Mg2+, while Zn2+ inhibited the system.

    Conclusions:

    • Streptococcus mutans possesses a specific, inducible phosphotransferase system for hexitol uptake and phosphorylation.
    • The system's regulation involves substrate induction and potentially a heat-stable stimulatory factor.
    • This metabolic capability may influence the bacterium's colonization and acid production in the oral environment.