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Purification and characterization of 3,3-dihydroxyazetidine from culture medium of Bacillus mesentericus and B.
Fumiko Ozone1, Yoshihiro Inoue, Akiko Shiraishi
1Department of Microbiology, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan.
Abstract:
A method is described for the purification of 3,3-dihydroxyazetidine (DHA), which accelerates the growth of Bifidobacterium spp., from the culture medium of Bacillus mesentericus (BM). Purification involved adsorption to an ion-exchange resin; it required less time and gave a higher yield than a previously reported method. Monitoring the inhibition of E. coli NIHJ JC-2, we searched for other strains that produced 3,3-dihydroxyazetidine. We found that not only B. mesentericus TO-A but also B. subtilis IFO13719 produced the compound of interest.
