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S Rous1, L Aubry

  • 1Département de Biochimie médicale, Université de Genève, Suisse, France

FEBS Letters
|March 22, 1971
PubMed
Summary
This summary is machine-generated.

Researchers isolated rat liver fatty acid synthetase from microsomes. Enzyme activity and electron microscopy confirmed it matches supernatant enzyme, suggesting microsomes

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Area of Science:

  • Biochemistry
  • Cell Biology

Background:

  • Fatty acid synthesis is crucial for cellular energy storage and membrane structure.
  • The location and regulation of fatty acid synthesis enzymes, like fatty acid synthetase (FAS), are key to understanding cellular metabolism.
  • Microsomes, a cellular fraction, have been implicated in various metabolic processes, but their specific role in de novo fatty acid synthesis requires clarification.

Purpose of the Study:

  • To isolate and characterize fatty acid synthetase (FAS) from rat liver microsomal fractions.
  • To compare the properties of microsomal FAS with that of FAS found in the cell's supernatant.
  • To elucidate the role of microsomes in the de novo synthesis of fatty acids within the cell.

Main Methods:

  • Isolation of fatty acid synthetase (FAS) from rat liver microsomal fractions.

Related Experiment Videos

  • Enzyme activity assays to quantify FAS catalytic function.
  • Electron microscopy (electromicrography) to analyze the structural characteristics of the purified enzyme.

    • Main Results:

    • Fatty acid synthetase (FAS) was successfully isolated from the microsomal fraction of rat liver.
    • Enzyme activity assays demonstrated comparable catalytic function between the isolated microsomal FAS and the supernatant FAS.
    • Electromicrography revealed that the purified microsomal FAS shares structural similarities with the supernatant enzyme.

    Conclusions:

    • The microsomal fraction contains fatty acid synthetase (FAS) that is biochemically and structurally identical to the enzyme found in the supernatant.
    • These findings support a role for microsomes in the de novo synthesis of fatty acids.
    • Microsomes may be involved in the cellular machinery for generating new fatty acids.