Related Experiment Video

Updated: Jul 11, 2026

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
05:31

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Published on: May 28, 2012

A colorimetric estimation of lipopolysaccharides

J Janda1, E Work

  • 1Department of Biochemistry, Imperial College, S.W.7, London, England

FEBS Letters
|September 1, 1971
PubMed
Summary

No abstract available in PubMed .

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Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...

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