Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

L Savu1, O Crepy, M A. Guerin

  • 1Laboratoire de Biochimie médicale (Laboratoire Associé no. 87 du CNRS), U.E.R. des Saints-Pères, 45 rue des Saints-Pères, 75--6ème, Paris, France

FEBS Letters
|April 15, 1972
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ForMAX - a beamline for multiscale and multimodal structural characterization of hierarchical materials.

Journal of synchrotron radiation·2024
Same author

Three-dimensional echocardiography and strain cardiac imaging in women with pre-eclampsia with follow-up to 6 months postpartum.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2023
Same author

Maternal cardiac function in twin pregnancy at 19-23 weeks' gestation.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2022
Same author

We report a patient who presented intrahepatic cholangitis and cholecystitis after SARS-CoV-2 infection.

Journal of gastroenterology and hepatology·2021
Same author

Three-dimensional echocardiography and cardiac strain imaging in women with gestational diabetes mellitus.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2021
Same author

[Incorporate hemodialysis patient's satisfaction to quality management].

Journal of healthcare quality research·2019
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
See all related articles

Rat embryo serum contains alpha(1)-foetoprotein, which binds oestrone and oestradiol-17beta with high affinity. This protein

Area of Science:

  • Biochemistry
  • Reproductive Biology
  • Endocrinology

Background:

  • Alpha(1)-foetoprotein (AFP) is a major serum protein found in early development.
  • Steroid hormone binding by serum proteins influences their bioavailability.
  • Understanding binding interactions is crucial for reproductive physiology.

Purpose of the Study:

  • To determine the binding affinity of rat alpha(1)-foetoprotein for oestrone and oestradiol-17beta.
  • To investigate the stoichiometry of binding.
  • To correlate binding characteristics with serum concentrations during pregnancy.

Main Methods:

  • Equilibrium binding studies were performed at 25°C.
  • Binding constants were calculated using established methods.
  • Serum concentrations were assessed during specific gestational periods.

Related Experiment Videos

Main Results:

  • Alpha(1)-foetoprotein exhibited similar binding constants (approximately 1 x 10(8) M(-1)) for both oestrone and oestradiol-17beta.
  • A binding stoichiometry of one binding site per mole of protein was suggested.
  • Elevated AFP levels in rat embryo serum between days 17-19 of gestation were observed.

Conclusions:

  • Rat alpha(1)-foetoprotein demonstrates significant binding affinity for key oestrogens.
  • The high concentration of AFP during late pregnancy contributes to high phenolsteroid fixation.
  • These findings highlight the role of AFP in modulating oestrogen levels during rat gestation.