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R Kleine1, J Schubert

  • 1Physiologisch-chemisches Institut der Martin-Luther-Universität Halle/S., 1, Hollystrasse, DDR

FEBS Letters
|May 15, 1972
PubMed
Summary
This summary is machine-generated.

Rat kidney microsomes contain a high molecular weight arylamidase-alkaline phosphatase complex. This complex can be dissociated by various enzymes or acidification, revealing distinct protein bands for arylamidase and alkaline phosphatase.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Molecular Biology

Background:

  • Rat kidney microsomes possess a high molecular weight complex containing arylamidase and alkaline phosphatase activities.
  • Understanding the dissociation of this complex is crucial for characterizing individual enzyme functions.

Purpose of the Study:

  • To investigate the dissociation of the high molecular weight arylamidase-alkaline phosphatase complex from rat kidney microsomes.
  • To identify the specific enzymes and conditions that can separate the arylamidase and alkaline phosphatase components.

Main Methods:

  • Treatment of the complex with various hydrolytic enzymes (trypsin, chymotrypsin, pronase, papain, lipase, neuraminidase, hyaluronidase).
  • Acidification of the complex to pH 5.0.
  • Analysis of dissociation products using polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE.

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Main Results:

  • Selective solubilization of arylamidase and alkaline phosphatase activities was observed with specific enzymes, showing distinct electrophoretic mobilities.
  • Acid treatment (pH 5.0) and lipase also led to dissociation, with acid hydrolases and lipase releasing a second arylamidase zone.
  • SDS treatment resulted in dissociation into a single protein fragment of approximately 205,000 molecular weight.

Conclusions:

  • The arylamidase-alkaline phosphatase complex can be dissociated by enzymatic and acidic treatments.
  • Different dissociation methods yield distinct protein fragments, suggesting complex structural organization.
  • Further characterization of these fragments is necessary to understand their specific roles.