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G Berthillier1, R Got

  • 1Laboratoire de Chimie Biologique, Faculté de Médecine 69, Lyon, France

FEBS Letters
|June 1, 1970
PubMed
Summary
This summary is machine-generated.

Rat liver microsomal glucokinase, an enzyme crucial for glycolysis, is allosterically regulated by ATP and AMP. This enzyme

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Area of Science:

  • Biochemistry
  • Enzymology
  • Metabolic Regulation

Background:

  • Microsomal glucokinase plays a key role in glucose metabolism within the liver.
  • Understanding its kinetic properties is essential for elucidating glycolytic pathway regulation.

Purpose of the Study:

  • To investigate the kinetic properties of rat liver microsomal glucokinase.
  • To determine the enzyme's regulatory mechanisms and its potential role in feedback control of glycolysis.

Main Methods:

  • Enzyme kinetics assays were performed on rat liver microsomes.
  • The effects of ATP and AMP on glucokinase activity were analyzed.

Main Results:

  • Rat liver microsomal glucokinase is inhibited by adenosine triphosphate (ATP).
  • Adenosine monophosphate (AMP) activates or de-inhibits the enzyme.
  • The enzyme exhibits characteristics of allosteric regulation.

Conclusions:

  • Microsomal glucokinase functions as a typical allosteric enzyme.
  • Its regulatory properties suggest a role in the feedback control of glycolysis.