Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

L Kerp1, S Steinhilber, D D. Schmidt

  • 1Medizinische Universitätsklinik Freiburg i. Br. und Biochemisches Laboratorium der Farbenfabriken Bayer A G, Wuppertal-Elberfeldt, West Germany

FEBS Letters
|June 1, 1970
PubMed
Summary
This summary is machine-generated.

Guinea-pig antisera recognize both insulin and proinsulin, with varying affinities. Proinsulin antisera, after removing insulin antibodies, are valuable for proinsulin immunoassays.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo labelling of the spleen with a red-fluorescent cell dye.

Journal of immunological methods·1993
Same author

Influence of 6-week, 6 days per week, training on pituitary function in recreational athletes.

British journal of sports medicine·1993
Same author

Glucose-induced water movement from the intracellular to the extracellular space and its influence on calculations of glucose metabolism.

European journal of clinical investigation·1993
Same author

[Pulsatile insulin secretion into the portal vein in liver cirrhosis].

Deutsche medizinische Wochenschrift (1946)·1993
Same author

A time-resolved fluoroimmunoassay for human insulin based on two monoclonal antibodies.

Journal of immunological methods·1993
Same author

Biometry with a video-genlock interface and a computer-based image-analyzing system: use as a TV-videopupillometer.

Biomedical instrumentation & technology·1993
Same journal

Identification of a Shiga toxin A-derived peptide internalized into Gb3 receptor-bearing cells via interaction with the Shiga toxin B subunit.

FEBS letters·2026
Same journal

The dual role of lectins in cancer-immunotherapy tools and therapeutic targets.

FEBS letters·2026
Same journal

Decoding the dynamic extracellular matrix in cancer-3D models and bioscaffolds rewire the rules of tumor progression.

FEBS letters·2026
Same journal

Extending the classical sequence-structure-function paradigm through protein dynamics and context-dependent behavior.

FEBS letters·2026
Same journal

α-Synuclein aggregation landscape from phase separation to neurotoxic intermediates.

FEBS letters·2026
Same journal

Modelling stem cell differentiation related processes-A practical overview for biologists.

FEBS letters·2026
See all related articles

Area of Science:

  • Immunology
  • Endocrinology
  • Biochemistry

Background:

  • Insulin and proinsulin are crucial hormones in glucose metabolism.
  • Characterizing antibody binding sites is essential for developing accurate immunoassays.

Purpose of the Study:

  • To analyze guinea-pig antisera against bovine insulin and proinsulin.
  • To determine binding site affinities and concentrations for insulin and C-chain specificity.
  • To evaluate the potential of proinsulin antisera for proinsulin immunoassay.

Main Methods:

  • Analysis of guinea-pig antisera using binding assays.
  • Determination of association constants (affinities) for antibody-antigen interactions.
  • Quantification of specific binding sites for insulin and the C-chain.

Related Experiment Videos

Main Results:

  • Both insulin and proinsulin antisera exhibited high (Ak(1)) and low (Ak(2)) affinity binding sites.
  • Antisera to proinsulin contained equal high-affinity sites for the C-chain and insulin.
  • Insulin contaminated with proinsulin did not yield C-chain specific antibodies.

Conclusions:

  • Guinea-pig antisera possess distinct binding site populations for insulin and proinsulin.
  • Proinsulin antisera, following absorption of insulin-specific antibodies, are suitable for proinsulin immunoassays.