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Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
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Comparison of linear gradient and displacement separations in ion-exchange systems

V Natarajan1, S Ghose, S M Cramer

  • 1Department of Chemical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.

Biotechnology and Bioengineering
|April 12, 2002
PubMed
Summary

Displacement chromatography offers higher resolution and throughput than gradient mode for challenging separations. Both modes are effective for simpler protein ion-exchange separations, but displacement excels in complex scenarios.

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