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Updated: Jul 29, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
Published on: September 5, 2013
Cluster analysis on multiple drugs susceptibility supplements genotyping of methicillin-resistant Staphylococcus
J Yoshida1, A Umeda, T Ishimaru
1Infection Control Commitee, Shimonoseki City Hospital, Shimonoseki, Japan.
Objective:
To evaluate the typing power of cluster analysis of antimicrobial susceptibility.
Methods:
Results of pulsed-field gel electrophoresis in 71 strains of methicillin-resistant Staphylococcus aureus were compared with cluster analysis of the diameter of growth inhibition in 11 drugs. Subjects were a consecutive series of patients (n = 71) from the wards and outpatient units of a community teaching hospital.
Results:
The cluster analysis took 2 to 3 seconds once the data were entered into a computer. The sensitivity, specificity, and accuracy of the cluster analysis were 76.3%, 58.3%, and 73.2%, respectively, using genotyping as the reference.
Conclusions:
The cluster analysis offered real-time epidemiologic data at minimal cost and labor, warranting its cost-effective role.
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