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Substrate spectrum of tyrocidine thioesterase probed with randomized peptide N-acetylcysteamine thioesters
Guiyang Xie1, Mahesh Uttamchandani, Grace Y J Chen
1Department of Chemistry and Biotechnology Research Institute, The Hong Kong University of Science and Technology, Kowloon, SAR, Hong Kong, China.
Bioorganic & Medicinal Chemistry Letters
|April 18, 2002
Abstract:
Apparent kinetic constants k(cat) and K(m) were determined for tyrocidine thioesterase (TycC TE) using randomized peptide N-acetylcysteamine thioesters as substrate analogues. The enzyme has been found to be adequately active for the synthesis of positional-scanning libraries for novel antibiotic screening with reduced k(cat)/K(m) in the range of 2 to 82 folds lower than that of the wild-type sequence