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[Depolymerization of high-molecular-weight chitosan by the enzyme preparation Celloviridine G20x]
A V Il'ina1, Iu V Tkacheva, V P Varlamov
1Bioinzheneriya Center, Russian Academy of Sciences, Moscow, 117312 Russia.
Prikladnaia Biokhimiia I Mikrobiologiia
|April 19, 2002
Abstract:
A low-molecular-weight water-soluble chitosan was obtained from high-molecular-weight crab chitosan using the enzyme preparation Celloviridine G20x. Optimum conditions for the enzymatic hydrolysis were designed. The reaction should be performed for 4 h in a sodium-acetate buffer (pH 5.2) at 55 degrees C and the enzyme to substrate ratio of 1:400. Fractional extraction of chitosan hydrolysate by aqueous ethanol (ethanol: distilled water) yielded fractions with molecular weights in the range 3.2-26.4 kDa.