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[Entomopathogenic bacteria Bacillus thuringiensis as producers of restriction endonucleases]
L I Puchkova1, G V Kalmykova, L I Burtseva
1State Research Center of Virology and Biotechnology Vector, Collection of Cultures of Microorganisms, Kol'tsovo, Novosibirsk Oblast', 630559 Russia.
Abstract:
A total of 115 collection strains of Bacillus thuringiensis, belonging to various subspecies, have been studied for the presence of DNA restriction-modification systems. Restriction endonucleases of 13 strains have been isolated and characterized. No considerable correlations between the taxonomic positions of the bacteria and the specificities of the endonucleases isolated have been detected. It is concluded that the enzymes with identical specificities are present in both the crystalliferous and acrystalliferous strains of the same subspecies.