Identification of GEI-14 as a candidate pro-crossover factor by TurboID in C. elegans

Previously, our lab used TurboID-based proximity labeling followed by mass spectrometry (MS) to identify proteins that interact with COSA-1 , a cyclin-like pro-crossover factor that localizes at meiotic crossover sites. Here, we investigated potential interactions of COSA-1 with the top 10 COSA-1 ::TurboID-MS candidates ranked by relative intensity. Beyond the established COSA-1 - CDK-2 interaction, AlphaFold 3 predicted strong binding solely between COSA-1 and GEI-14 . Yeast two-hybrid assays confirmed this interaction and further revealed binding of GEI-14 to MSH-5 , another pro-crossover factor. These results demonstrate TurboID's utility in studying the pro-crossover interactome and highlight GEI-14 as a promising candidate for further characterization.