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[Group-specific antibodies against zearalenone and its metabolites and synthetic analogs]
A A Burkin1, G P Kononenko, N A Soboleva
1All-Russia Research Institute of Veterinary Sanitation, Hygiene, and Ecology, Russian Academy of Agricultural Sciences, Moscow, 123022 Russia.
Abstract:
Formation kinetics, specificity, and analytical potential of polyclonal antibodies raised in rabbits against BSA conjugates of carboxymethyloxime-zearalanone (CMO-ZAN) and carboxymethyloxime-zearalenone (CMO-ZEN). Preparation of the conjugates involved conversion of CMO-ZAN and CMO-ZEN into activated esters and carbodiimide condensation. Two versions of a group-specific enzyme immunoassay (for zearalenone/alpha-zearalenone and zearalanol/alpha-zearalanol) based on heterologous combination of solid-phase antigens are described (sensitivity, 0.01 ng/ml).
