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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 19, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Effective generation of transgenic pigs and mice by linker based sperm-mediated gene transfer.

Keejong Chang1, Jin Qian, MeiSheng Jiang

  • 1BioAgri Corporation-Taiwan Branch, Fl. 8-8, No. 8, Song-Chiang Rd., Taipei, Taiwan. kchang@bioagricorp.com.tw

BMC Biotechnology
|April 20, 2002
PubMed
Summary

A novel linker-based sperm-mediated gene transfer (LB-SMGT) method significantly enhances the efficiency of producing large transgenic animals. This technique shows high success rates in pigs and mice, offering broad applicability across species.

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Area of Science:

  • Animal biotechnology
  • Reproductive biology
  • Genetics

Background:

  • Transgenic animals are crucial research and applied tools.
  • Microinjection, the current standard for transgenic mouse production, has limited success in larger species.
  • A new method, linker-based sperm-mediated gene transfer (LB-SMGT), is introduced to improve efficiency.

Purpose of the Study:

  • To develop and validate a novel method for efficient transgenic animal production.
  • To demonstrate the effectiveness of LB-SMGT across multiple species.
  • To improve the efficiency of generating large transgenic animals.

Main Methods:

  • Utilized a linker protein (monoclonal antibody C) that binds to sperm surface antigens and DNA.
  • Developed a linker-based sperm-mediated gene transfer (LB-SMGT) technique.
  • Tested LB-SMGT in pigs and mice, analyzing germ-line transmission and transgene expression.

Main Results:

  • The linker protein (mAb C) demonstrated reactivity with sperm across diverse species including pigs, mice, chickens, cows, goats, sheep, and humans.
  • LB-SMGT achieved high germ-line transfer rates: 37.5% in pigs and 33% in mice.
  • Transgene expression was confirmed in 61% of F0 transgenic pigs.

Conclusions:

  • LB-SMGT offers a highly efficient and broadly applicable method for generating transgenic animals.
  • This technique overcomes limitations of traditional methods like microinjection, especially for larger species.
  • LB-SMGT holds significant potential for advancing animal biotechnology across numerous species.