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Related Experiment Videos

Evaluation of a selective medium for Brucella isolation using natamycin.

J A Stack1, M Harrison, L L Perrett

  • 1Veterinary Laboratories Agency, Addlestone, Surrey, UK.j.a.stack@vla.defra.gsi.gov.uk

Journal of Applied Microbiology
|April 23, 2002
PubMed
Summary

Natamycin is a suitable replacement for the unavailable anti-fungal agent cycloheximide in Brucella isolation media. It effectively inhibits contaminants while allowing Brucella growth.

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Area of Science:

  • Microbiology
  • Veterinary Medicine

Background:

  • Cycloheximide, an anti-fungal agent used in Brucella isolation media, is now globally unavailable.
  • Brucella isolation requires selective media to inhibit fungal and other microbial contaminants.

Purpose of the Study:

  • To identify and evaluate a suitable anti-fungal agent to replace cycloheximide in Brucella isolation media.
  • To assess the efficacy of natamycin as an alternative anti-fungal agent.

Main Methods:

  • Evaluation of natamycin using 28 Brucella isolates, 18 yeasts, and 14 fungi.
  • Testing on bovine milk samples, vaginal swabs, and artificially infected milk samples.
  • Comparison of Brucella recovery and contaminant inhibition with natamycin versus cycloheximide.

Main Results:

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  • Natamycin effectively inhibited the growth of yeasts, fungi, and other bacteria.
  • Brucella recovery from artificially inoculated milk samples increased with natamycin-containing media.
  • Both natamycin and cycloheximide allowed growth of Brucella isolates and inhibited most fungi.

Conclusions:

  • Natamycin is a suitable and effective alternative to cycloheximide for Brucella isolation.
  • The use of natamycin maintains Brucella growth while eliminating common contaminants.