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[The phospholipase A2 inhibitor, aristolochic acid, inhibits chloride secretion without altering barrier function]
Aim:
To study the effect of aristolochic acid (AA) and the effect of phospholipase A2 (PLA2) on barrier function and electrogenic chloride secretion in intestinal epithelium.
Material And Methods:
Electrophysiological studies were performed in the T84 cell line and rat distal colon. Ionic secretion and transepithelial resistance were determined.
Results:
Exogenous AA increased calcium-stimulated secretion in the T84 cell line. Incubation of rat distal colon in the presence of AA, a PLA2 inhibitor, reduced basal ionic secretion without affecting transepithelial resistance and inhibited ionic secretion stimulated by a cyclic adenosine monophosphate (AMPc) agonist, forskolin. In T84 cells, AA inhibited both carbachol- and forskolin-stimulated secretion.
Conclusion:
PLA2 modulates electrogenic chloride secretion but has no effect on barrier function in the T84 cell line or in rat distal colon.