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Related Experiment Videos

Proton-gated channels in PC12 cells.

Xiang-Ping Chu1, Jennifer Miesch, Martha Johnson

  • 1Robert S. Dow Neurobiology Laboratories, Legacy Research, Portland, Oregon 97232, USA.

Journal of Neurophysiology
|April 27, 2002
PubMed
Summary

PC12 cells express functional acid-sensing ion channels (ASICs), specifically homomeric ASIC1a. This neuronal cell line offers a valuable model for studying ASIC1a

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Zinc Permeation Through Acid-Sensing Ion Channels.

Cells·2026

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Ion Channel Physiology

Background:

  • Acid-sensing ion channels (ASICs) are crucial in neuronal function, but their roles are unclear due to complex subunit interactions.
  • Identifying neuronal models expressing single ASIC populations is vital for functional studies.

Purpose of the Study:

  • To investigate the presence and function of ASICs in PC12 cells, a neuronal cell line.
  • To determine if PC12 cells can serve as a model for studying specific ASIC subunits.

Main Methods:

  • Patch-clamp electrophysiology to record ion channel activity.
  • Calcium imaging to assess cellular responses.
  • Reverse transcription polymerase chain reaction (RT-PCR) to detect gene expression.

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Main Results:

  • PC12 cells exhibited transient inward currents upon exposure to acidic pH, indicating functional ASICs.
  • These channels showed selectivity for Na+ with notable Ca2+ permeability and were blocked by amiloride.
  • The specific ASIC1a blocker PcTX1 inhibited currents, and RT-PCR confirmed ASIC1a transcript expression.

Conclusions:

  • PC12 cells predominantly express functional homomeric ASIC1a channels.
  • This cell line represents an ideal model for investigating the physiological and pathological roles of ASIC1a.