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[Experimental study on the change and effect of TGF-beta 1 after skin expansion]
Kai Liu1, Zhihong Fan, Yunliang Qian
1Department of Plastic Surgery, Ninth Hospital of Shanghai, Second Medical University, Shanghai 200011, China.
Objective:
To study the injury process of the skin due to expansion.
Methods:
New Zealand rabbits were divided into 4 groups: rapid expansion and slow expansion as well as two control groups. The changes of skin TGF-beta 1 were observed immediately after expansion and at 1, 12, 24 weeks after expansion. Immunohistochemistry and in situ hybridization technique were applied.
Results:
TGF-beta 1 increased in the skin immediately after expansion. In groups of rapid expansion, TGF-beta 1 increased faster and then decreased also faster than slow expansion groups. The results from immunohistochemistry and in situ hybridization were almost same.
Conclusion:
Expansion resulted in skin injury. Rapid expansion injured the skin more seriously than slow expansion. TGF-beta 1 may be the main regulating factor to repairing process.