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Related Experiment Videos

Proton magnetization transfer effect in rat liver lactate.

Tom Dresselaers1, Niki Bergans, Paul Van Hecke

  • 1Biomedische NMR-Eenheid, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium.

Magnetic Resonance in Medicine
|April 30, 2002
PubMed
Summary

Off-resonance magnetization transfer (MT) experiments reveal lactate methyl protons in rat liver. Lactate NMR visibility increases during ischemia, suggesting macromolecular mediation of proton coupling.

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Area of Science:

  • Biophysics
  • Magnetic Resonance Imaging
  • Metabolic Studies

Background:

  • Lactate is a key metabolite in liver function and disease.
  • Understanding lactate's NMR visibility is crucial for in vivo studies.
  • Magnetization transfer (MT) can probe interactions between different proton pools.

Purpose of the Study:

  • To investigate lactate methyl proton behavior using off-resonance MT.
  • To quantify lactate pool sizes in perfused and ischemic rat livers.
  • To explore the interaction between lactate and water protons.

Main Methods:

  • In situ rat liver experiments using off-resonance lactate MT.
  • Application of a two-pool model for lactate pool size estimation.
  • Analysis of MT data using superLorentzian and Gaussian lineshapes.

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Main Results:

  • Significant MT effect observed for lactate methyl protons.
  • Increased MT effect in ischemic livers compared to perfused livers.
  • Estimated lactate pool size of ~1% in perfused and 1.8-2.5% in ischemic livers.
  • SuperLorentzian lineshape provided a better fit for MT data.
  • Evidence of coupling between lactate methyl and water protons.

Conclusions:

  • Lactate in rat liver is nearly fully NMR-visible.
  • Ischemia leads to an increase in the motionally restricted lactate pool.
  • Macromolecular mediation is a likely mechanism for lactate-water proton coupling.