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Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis
1Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic. horka@iach.cz
Electrophoresis
|May 1, 2002
Abstract:
Pyrenebutanoate as the amphiphilic fluorescent compound is suggested as a buffer additive in capillary zone electrophoresis (CZE) for a dynamic modification of several protein samples. Using deuterium lamp UV excitation for the on-column fluorometric detection, minimum detectable amounts in the amol-range of the proteins sampled on the CZE capillary was achieved.