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Identification of radical scavengers in sweet grass (Hierochloe odorata)
Audrius Pukalskas1, Teris A van Beek, Rimantas P Venskutonis
1Department of Food Technology, Kaunas University of Technology, Radvilenu pl. 19, LT-3028 Kaunas, Lithuania.
Abstract:
Extracts from aerial parts of sweet grass (Hierochloe odorata) were active DPPH free radical scavengers. The active compounds were detected in extract fractions using HPLC with on-line radical scavenging detection. After multistep fractionation of the extract, two new natural products possessing radical scavenging activity were isolated, and their structures were elucidated by NMR and MS. They were identified as 5,8-dihydroxybenzopyranone and 5-hydroxy-8-O-beta-D-glucopyranosyl-benzopyranone. Activities of the compounds isolated were tested by DPPH and ABTS free radical scavenging assays, and compared with the known natural antioxidant rosmarinic acid and Trolox.