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[Oxidized LDL, clinical aspects and measurement methods]
1Division of Clinical Preventive Medicine, Niigata University Graduate School of Medical and Dental Science, Niigata 951-8510.
Rinsho Byori. the Japanese Journal of Clinical Pathology
|May 3, 2002
Summary
Oxidative modification of low-density lipoprotein (LDL) accelerates atherosclerosis. Evaluating LDL oxidation in vitro and its clinical applications are crucial for developing new lipid-lowering and antioxidant therapies.
Area of Science:
- Biochemistry
- Cardiovascular Science
- Medical Diagnostics
Context:
- Oxidative modification of low-density lipoprotein (LDL) is a key process in atherogenesis.
- Current methods for evaluating LDL oxidation in vitro include assessing lipid peroxidation products, surface charge, and spectrophotometric patterns.
- The lag time of LDL oxidation is a potential marker for evaluating drug efficacy.
Purpose:
- To review methods for evaluating LDL oxidation in vitro.
- To discuss the potential of enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies for clinical assessment of LDL oxidation.
- To highlight the challenges in establishing clinical applicability due to antibody variability.
Summary:
- Various methods exist to evaluate in vitro low-density lipoprotein (LDL) oxidation, including analysis of lipid peroxidation products, surface charge, and spectrophotometric patterns.
- The lag time of LDL oxidation shows promise for assessing the effectiveness of lipid-lowering and antioxidant drugs.
- Enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies offers potential for clinical use, but standardization is needed due to antibody diversity.
Impact:
- Improved understanding of LDL oxidation mechanisms and their role in atherosclerosis.
- Potential for developing more effective diagnostic tools for cardiovascular disease risk assessment.
- Facilitation of drug development for lipid-lowering and antioxidant therapies targeting atherosclerosis.