Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Splicing deficiency is driven by genomic erosion in non-recombining algal mating-type chromosomes.

PLoS biology·2026
Same author

Extensive splicing deficiency in a degenerating mating-type chromosome.

bioRxiv : the preprint server for biology·2025
Same author

Comprehensive analysis of Saccharomyces cerevisiae intron structures in vivo.

Nature structural & molecular biology·2025
Same author

Corrigendum: Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: application to direct RNA sequencing on nanopores.

RNA (New York, N.Y.)·2025
Same author

Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers.

PLoS genetics·2024
Same author

Intron lariat spliceosomes convert lariats to true circles: implications for intron transposition.

Genes & development·2024

Related Experiment Video

Updated: Jul 18, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays.

Tyson A Clark1, Charles W Sugnet, Manuel Ares

  • 1Department of Molecular, Cell, and Developmental Biology, Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA.

Science (New York, N.Y.)
|May 4, 2002
PubMed
Summary

Researchers mapped yeast gene splicing using microarrays to study mRNA processing factors. Key factors like Prp17p and Prp18p are essential for removing introns, particularly those with short distances.

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Related Experiment Videos

Last Updated: Jul 18, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Introns are non-coding sequences within eukaryotic genes.
  • Messenger RNA (mRNA) splicing removes introns to form mature mRNA.
  • The precise mechanisms of genome interpretation during mRNA splicing are not fully understood.

Purpose of the Study:

  • To investigate the genomewide effects of mRNA processing factors on splicing.
  • To identify functional relationships between different mRNA processing factors.
  • To understand gene-specific dependencies on mRNA processing.

Main Methods:

  • Development of custom microarrays to differentiate spliced and unspliced RNA.
  • Genomewide analysis of splicing patterns upon depletion of 18 mRNA processing factors in yeast.
  • Utilizing gene-specific indexes to account for transcription and decay rates.
  • Confirmation of array findings using quantitative polymerase chain reactions (qPCR).

Main Results:

  • Identified functional relationships between mRNA processing factors based on their shared effects on RNA splicing.
  • Revealed distinct groups of genes exhibiting differential dependencies on specific mRNA processing factors.
  • Confirmed that Prp17p and Prp18p are crucial for the removal of introns with short branchpoint-to-3' splice site distances.

Conclusions:

  • The study provides a genomewide map of mRNA processing factor functions in yeast splicing.
  • Common effects on spliced and unspliced RNA reveal intricate functional networks among processing factors.
  • Specific factors, Prp17p and Prp18p, play essential roles in splicing short introns, highlighting mechanistic insights.