A simple method for the prediction of ABO incompatibility using Sephadex A-50. Prediction of ABO incompatibility

A rapid and simple method of demonstrating the presence of high titre IgG anti-A or anti-B (anti A/B) is described. The method requires the separation of IgG anti-A/B from IgM anti-A/B using DEAE Sephadex A-50 by a simple spin technique, and the subsequent titration of the IgG fraction to detect the presence of high titre IgG anti-A/B. Since high titre IgG anti-A/B is usually associated with ABO incompatibility the test is a useful guide to the prediction of ABO incompatibility in routine antenatal samples.