Related Experiment Video
Updated: Oct 1, 2026

Determination of the Photoisomerization Quantum Yield of a Hydrazone Photoswitch
Published on: February 7, 2022
Multiwavelength spectrophotometric resolution of the micro-equilibria of cetirizine
Abstract:
The acid-base properties of the antihistamine (H1 receptor antagonist) cetirizine have been studied by using a previously developed multiwavelength spectrophotometric titration method. A new computational procedure called "Two-Step-Divide-and-Conquer" (TSDC), which applied evolving factor analysis (EFA) and target factor analysis (TFA), has been derived to unravel the micro-equilibria of the triprotic zwitterionic compound from the spectral data. We have demonstrated that a single spectrophotometric titration experiment is sufficient to determine the 12 unknown microconstants and the distribution of microspecies, which are in good agreement with literature values, where available.
More Related Videos
05:46Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Published on: August 1, 2018
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Related Concept Videos
Chromatographic Resolution
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar absorptivity (ε) or log ε on the y-axis (ordinate)...
UV–Vis Spectrometers
High-Resolution Mass Spectrometry (HRMS)
Chemical Ionization (CI) Mass Spectrometry
Racemic Mixtures and the Resolution of Enantiomers