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In vitro behaviour of human osteoblasts on dentin and bone
B A A Scheven1, D Marshall, R M Aspden
1Department of Orthopaedic Surgery, University of Aberdeen, Polwarth Building Foresterhill, AB25 2ZD, Scotland, UK. b.scheven@abdn.ac.uk
Cell Biology International
|May 7, 2002
Summary
Osteoblasts, including primary human osteoblast-like cells (HOBs) and cell lines, show distinct behaviors on dentin compared to standard plastic. Primary cells exhibit better attachment and viability on dentin, while cell lines proliferate slower.
Area of Science:
- Biomaterials Science
- Cell Biology
- Tissue Engineering
Background:
- Osteoblast behavior is crucial for bone regeneration and understanding cell-substrate interactions.
- Standard tissue culture polystyrene is commonly used, but natural materials like dentin may offer different cellular responses.
Purpose of the Study:
- To compare the behavior of osteoblasts on dentin versus standard tissue culture polystyrene.
- To investigate differences between primary human osteoblast-like cells (HOBs) and human osteosarcoma cell lines (MG63, HOS TE85, SaOS-2).
Main Methods:
- Osteoblast cell lines and primary HOBs were cultured on untreated plastic, roughened plastic, and dentin slices.
- Cellular attachment, viability, proliferation, morphology, and alkaline phosphatase (AP) activity were assessed.
Main Results:
- Primary HOBs showed faster attachment, better viability, and more cellular extensions on dentin compared to cell lines.
- Cell lines proliferated slower on dentin than plastic, while primary HOBs' growth was unaffected.
- Alkaline phosphatase activity initially increased in primary cells on dentin but decreased after longer culture periods for all cell types.
Conclusions:
- Osteoblastic cells exhibit differential responses to natural calcified substrates (dentin) compared to standard culture plastic.
- Significant variations in cellular behavior exist between primary HOBs and continuous osteosarcoma cell lines on different substrates.