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CD44 variant exons in leukemia and lymphoma
Elif Akisik1, Sevil Bavbek, Nejat Dalay
1Istanbul University, Department of Basic Oncology, Oncology Institute Capa, Istanbul, 34390, Turkey.
Pathology Oncology Research : POR
|May 8, 2002
Summary
The CD44v6-v10 variant, a form of CD44 (a cell surface glycoprotein), was found in patients with hematologic malignancies but not in healthy individuals. This suggests CD44v6-v10 may serve as a marker for these diseases.
Area of Science:
- Molecular Biology
- Oncology
- Hematology
Background:
- CD44 is a cell surface glycoprotein involved in lymphocyte activation, cell adhesion, and migration.
- Standard CD44 (CD44S/H) is the hematopoietic form, while variant isoforms (CD44v1-v10) arise from additional exons.
- Elevated CD44v6 expression is linked to metastasis in some cancers.
Purpose of the Study:
- To investigate CD44v6 and v6-containing variant expression in chronic myeloid leukemia and lymphoma.
- To evaluate CD44 variant expression as a potential diagnostic marker for hematologic malignancies.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect CD44 exon v6 and variant transcripts.
- Expression levels were analyzed in patients with chronic myeloid leukemia and lymphoma, and compared to healthy controls.
Main Results:
- CD44 exon v6 was detected in all patients and controls.
- CD44v6-v10 mRNA was present in 25 patients but absent in the control group.
- Specific transcripts like CD44v6/v9-10, CD44v6-v7, and CD44v6/v10 were detected in varying numbers of patients.
Conclusions:
- The expression of CD44v6-v10 variants appears to be associated with hematologic malignancies.
- CD44v6-v10 may serve as a potential molecular marker for diagnosing and understanding these diseases.