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The let-7 microRNA binding site variant in KRAS as a predictive biomarker for head and neck cancer patients with

Murat Ulusan1, Sena Sen2, Rasim Yilmazer1

  • 1Department of Otorhinolaryngology-Head and Neck Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Türkiye.

Pathology, Research and Practice
|October 13, 2022
PubMed
Abstract

Insights

The KRAS-LCS6 variant, a let-7 miRNA binding site polymorphism, is not linked to head and neck cancer (HNC) risk. However, this variant may predict lymph node metastasis in HNC patients.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • The let-7 miRNA family regulates oncogenes like KRAS, impacting carcinogenesis.
  • Polymorphic variations in miRNA binding sites can influence cancer development and progression.

Purpose of the Study:

  • To investigate the KRAS-LCS6 variant (rs61764370) in the 3'UTR of the KRAS gene.
  • To assess its potential as a predictive biomarker for head and neck cancer (HNC).
  • To evaluate associations between the KRAS-LCS6 variant and HNC clinicopathological parameters.

Main Methods:

  • Genotyping of the KRAS-LCS6 variant in 216 Turkish HNC patients and 85 healthy controls.
  • DNA extraction from whole blood followed by polymerase chain reaction and restriction fragment length polymorphism analysis.
  • Evaluation of genotype and allele frequencies using De-Finetti case-control program and univariate/Cox regression analyses.

Main Results:

  • The KRAS-LCS6 variant was not associated with overall HNC risk (p > 0.05).
  • The homozygous variant allele showed a significant association with lymph node metastasis in HNC patients (OR=2.370, p=0.03).
  • Genotype frequencies were significantly associated with gender, smoking status, alcohol consumption, disease stage, and lymph node metastasis (p < 0.05).

Conclusions:

  • This study is the first to demonstrate a link between the KRAS-LCS6 variant and lymph node metastasis in HNC.
  • The KRAS-LCS6 variant may serve as a potential predictive biomarker for lymph node metastasis in head and neck cancer.
Keywords:
head and neck cancerkras polymorphismlcs-6lymph node metastasisrestriction fragment length polymorphism

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