Related Experiment Videos
A simple method for enrichment of uninucleate conidia of Aspergillus oryzae
Seiichi Hara1, Ryohei F Tsuji, Osamu Hatamoto
1Noda Institute for Scientific Research, Noda-shi, Chiba, Japan. shara@mail.kikkoman.co.jp
Bioscience, Biotechnology, and Biochemistry
|May 15, 2002
Abstract:
Aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. Analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. Based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. Our results also suggest that the method is useful for elimination of heterokaryons.