Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Maize tertiary trisomic stocks derived from B-A translocations.

D L Auger1, J A Birchler

  • 1Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.

The Journal of Heredity
|May 16, 2002
PubMed
Summary

Researchers developed novel tertiary trisomic stocks in maize using B-A translocations to study chromosome dosage manipulation. These stocks allow for comparing maternal and paternal transmission of B-A chromosomes, providing valuable genetic tools.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanisms of Mendelian dominance.

Clinical genetics·2017
Same author

Histone phosphorylation: its role during cell cycle and centromere identity in plants.

Cytogenetic and genome research·2014
Same author

Accumulation of multiple copies of maize minichromosomes.

Cytogenetic and genome research·2012
Same author

Recent and frequent insertions of chloroplast DNA into maize nuclear chromosomes.

Cytogenetic and genome research·2010
Same author

Diversity of chromosomal karyotypes in maize and its relatives.

Cytogenetic and genome research·2010
Same author

Interaction study of the male specific lethal (MSL) complex and trans-acting dosage effects in metafemales of Drosophila melanogaster.

Cytogenetic and genome research·2009

Area of Science:

  • Plant Genetics
  • Chromosome Engineering
  • Maize Biology

Background:

  • Reciprocal translocations between B chromosomes and A chromosomes in maize offer tools for chromosomal segment dosage manipulation.
  • B-A translocations are instrumental in generating aneuploid gametes.

Purpose of the Study:

  • To develop and characterize tertiary trisomic stocks derived from B-A reciprocal translocations in maize.
  • To compare the effects of maternally versus paternally transmitted B-A chromosomes.
  • To analyze the transmission rates of B-A chromosomes in tertiary trisomic stocks.

Main Methods:

  • Development of 15 B-A reciprocal translocation stocks with genetic markers.
  • Creation of tertiary trisomic stocks (2n+1 aneuploids) utilizing B-A translocation elements.

Related Experiment Videos

  • Introgression of stocks into the W22 inbred maize line for comparative analysis.
  • Assessment of male and female transmission rates of B-A chromosomes.

    • Main Results:

    • Successfully generated tertiary trisomic stocks from B-A translocations.
    • Tertiary trisomics efficiently transmit hyperploid gametes maternally, contrasting with sperm aneuploidy from B-A translocations.
    • Data on male and female transmission rates of B-A chromosomes in these stocks are presented.

    Conclusions:

    • Tertiary trisomic stocks derived from B-A translocations are effective tools for studying chromosome dosage and transmission.
    • The study provides a foundation for comparing maternal and paternal effects of B-A chromosome transmission in maize.
    • These genetic resources enhance the manipulation and analysis of chromosomal segments in maize.