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Solid-phase extraction procedure to remove organic acids from honey.

Silvia Suárez-Luque1, Inés Mato, José F Huidobro

  • 1Facultad de Farmacia, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago, Santiago de Compostela, Galicia, Spain.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|May 16, 2002
PubMed
Summary

A new solid-phase extraction method efficiently isolates organic acids from honey. This optimized procedure ensures high recovery rates for accurate analysis of honey composition.

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Area of Science:

  • Food Chemistry
  • Analytical Chemistry
  • Separation Science

Background:

  • Honey composition analysis is crucial for quality control.
  • Organic acids are key components influencing honey's flavor and properties.
  • Existing methods for organic acid extraction from honey can be complex and time-consuming.

Purpose of the Study:

  • To develop and optimize a solid-phase extraction (SPE) method for isolating organic acids from honey.
  • To achieve high recovery rates for target organic acids while minimizing interfering substances.
  • To establish a reliable analytical procedure for honey quality assessment.

Main Methods:

  • Utilized an anion-exchange cartridge for the selective isolation of organic acids.
  • Optimized parameters including cartridge activation, sample loading, washing, and elution.

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  • Employed sodium hydroxide for activation and sulfuric acid for elution of organic acids.
  • Analyzed eluted acids directly using chromatography.

    • Main Results:

    • Achieved high recovery rates for standard organic acid solutions (99.2–103.4%).
    • Demonstrated satisfactory recovery rates for organic acids in actual honey samples (62.9–99.4%).
    • The optimized SPE procedure effectively separated target organic acids from honey matrix interferences.

    Conclusions:

    • The developed solid-phase extraction method is effective for the quantitative analysis of organic acids in honey.
    • This technique offers a reliable and efficient approach for honey quality and authenticity testing.
    • The optimized procedure provides a valuable tool for researchers and food industry professionals.