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Protective effect of pretreatment with low-dose lipopolysaccharide on D-galactosamine-induced acute liver failure
Toru Kono1, Hiromi Kotani, Toshiyuki Asama
1Second Department of Surgery, Asahikawa Medical College, Hokkaido, Japan. kono@asahikawa-med.ac.jp
Background And Aims:
The effect of low-dose lipopolysaccharide (LPS) induced nitric oxide (NO) on liver damage and survival in rats with acute liver failure caused by a lethal dose of D-galactosamine (D-gal) was studied.
Results:
Ninety percent of control animals died within 4 days after D-gal injection, but pretreatment with low-dose LPS significantly decreased mortality to 5%. There was marked elevation in serum aspartate aminotransferase and alanine aminotransferase levels 24 h after D-gal injection. These aminotransferases were significantly improved in low-dose LPS pretreated rats 24 h after the administration of D-gal. NG-Nitro-L-arginine-methyl ester, but not NG-nitro-D-arginine-methyl ester, reversed this cytoprotection.
Conclusion:
Pretreatment with low-dose LPS prevents experimental liver failure induced by D-gal through activation of endogenous NO synthesis.
