Related Experiment Video

Updated: Aug 19, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Overexpression of the sis oncogene in a canine osteosarcoma cell line

R A Levine1

  • 1Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. ral1@cornell.edu

Veterinary Pathology
|May 17, 2002
PubMed

Insights

Researchers identified the sis oncogene in canine osteosarcoma cell lines, suggesting a role for platelet-derived growth factor (PDGF) in tumor development. This discovery may lead to new therapeutic strategies for canine osteosarcoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Canine Cancer Research

Background:

  • Specific oncogenes driving canine osteosarcoma (OS) pathogenesis remain unidentified.
  • Understanding the molecular mechanisms of OS is crucial for developing targeted therapies.

Purpose of the Study:

  • To identify specific oncogenes involved in canine osteosarcoma.
  • To investigate the role of platelet-derived growth factor (PDGF) signaling in canine OS.

Main Methods:

  • Characterization of four canine osteosarcoma cell lines.
  • Analysis of sis oncogene and c-myc expression levels.
  • Assessment of PDGF receptor activation in response to conditioned medium.

Main Results:

  • One cell line (CO8) overexpressed the sis oncogene, encoding PDGF-beta.
  • Elevated expression of c-myc, a downstream target of PDGF signaling, was observed.
  • Conditioned medium from CO8 induced PDGF receptor activation in murine 3T3 cells, indicating functional PDGF signaling.

Conclusions:

  • The sis oncogene and subsequent PDGF autocrine loop may contribute to the pathogenesis of a subset of canine osteosarcomas.
  • Canine osteosarcoma cell lines possess functional PDGF receptors, making them responsive to PDGF.
  • This finding opens avenues for exploring PDGF-targeted therapies in canine osteosarcoma.

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...