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Updated: Jan 31, 2026

Essential Components of Borreliella Borrelia burgdorferi In Vitro Transcription Assays
Published on: July 22, 2022
TFIIH plays an essential role in RNA polymerase I transcription
Sebastian Iben1, Herbert Tschochner, Mirko Bier
1Division of Molecular Biology of the Cell II, German Cancer Research Center, D-69120, Heidelberg, Germany.
Abstract:
TFIIH is a multisubunit protein complex that plays an essential role in nucleotide excision repair and transcription of protein-coding genes. Here, we report that TFIIH is also required for ribosomal RNA synthesis in vivo and in vitro. In yeast, pre-rRNA synthesis is impaired in TFIIH ts strains. In a mouse, part of cellular TFIIH is localized within the nucleolus and is associated with subpopulations of both RNA polymerase I and the basal factor TIF-IB. Transcription systems lacking TFIIH are inactive and exogenous TFIIH restores transcriptional activity. TFIIH is required for productive but not abortive rDNA transcription, implying a postinitiation role in transcription. The results provide a molecular link between RNA polymerase I transcription and transcription-coupled repair of active ribosomal RNA genes.
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