[Establishment of sandwich ELISA with McAbs to measure histidine-rich glycoprotein in plasma]

The objective of this paper is to establish a method using sandwich ELISA with McAbs to measure plasma histidine-rich glycoprotein (HRG). The results showed that this method, with a working range from 2.5 ng.ml-1 to 80 ng.ml-1, provided the lower limit of detection of 1 ng.ml-1, the average intra-assay CV, the interassay CV and the recovery rate of HRG were 8.2%, 11.5% and 89.1%, respectively. Interference by antithrombin III and human albumin was not significant. With this method, the concentration of plasma HRG from healthy donors was (110.3 +/- 9.7) mg.L-1, and the value of plasma HRG in liver cirrhosis was (79.5 +/- 12.6) mg.L-1. These results suggest that sandwich ELISA with McAbs is high sensitive, precise and specific.